Transcript: Human NM_133374.2

Homo sapiens zinc finger protein 618 (ZNF618), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
ZNF618 (114991)
Length:
9133
CDS:
100..2685

Additional Resources:

NCBI RefSeq record:
NM_133374.2
NBCI Gene record:
ZNF618 (114991)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_133374.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000436359 GACAGTGTACGTGACGGATTG pLKO_005 1656 CDS 100% 6.000 8.400 N ZNF618 n/a
2 TRCN0000433517 GTCAGGAAAGCTGAGCGATTG pLKO_005 3051 3UTR 100% 6.000 8.400 N ZNF618 n/a
3 TRCN0000432852 AGCGCTTTCAGTCGGAGAGTA pLKO_005 910 CDS 100% 4.950 6.930 N ZNF618 n/a
4 TRCN0000137602 CAAGAGCTATGTGCTTGGTGT pLKO.1 1548 CDS 100% 2.640 3.696 N ZNF618 n/a
5 TRCN0000137766 GCCAATAACACCACTACCAGT pLKO.1 1159 CDS 100% 2.640 3.696 N ZNF618 n/a
6 TRCN0000136106 CACATCAAGAGCTATGTGCTT pLKO.1 1543 CDS 100% 0.264 0.370 N ZNF618 n/a
7 TRCN0000416159 GTGTGAACAAGCGCTTCTAAT pLKO_005 2595 CDS 100% 13.200 10.560 N ZNF618 n/a
8 TRCN0000431547 GACCCTTGGGTCTTACGAATG pLKO_005 429 CDS 100% 6.000 4.800 N ZNF618 n/a
9 TRCN0000429800 ACACTGTGGACCTCATTATAA pLKO_005 2781 3UTR 100% 15.000 10.500 N ZNF618 n/a
10 TRCN0000422910 AGTCCAGAAGATATGAATAAA pLKO_005 2635 CDS 100% 15.000 10.500 N ZNF618 n/a
11 TRCN0000138191 CCAGAGGAACAGTGCCAATAA pLKO.1 1146 CDS 100% 13.200 9.240 N ZNF618 n/a
12 TRCN0000437785 GAGCCTCCCAAAGCAACAATT pLKO_005 539 CDS 100% 13.200 9.240 N ZNF618 n/a
13 TRCN0000432626 GGTGTGCAGATTGGCAGAAAG pLKO_005 3135 3UTR 100% 10.800 7.560 N ZNF618 n/a
14 TRCN0000430581 TGCTGTCGGAGTTCGTGATGT pLKO_005 1625 CDS 100% 4.950 3.465 N ZNF618 n/a
15 TRCN0000138008 GAAGATGATCGGCTAGGCAAA pLKO.1 2413 CDS 100% 4.050 2.835 N ZNF618 n/a
16 TRCN0000138804 GCTCAAGGAGAACTTCAAGGT pLKO.1 2193 CDS 100% 2.640 1.848 N ZNF618 n/a
17 TRCN0000138324 CAAGGAGAACTTCAAGGTGCA pLKO.1 2196 CDS 100% 2.160 1.512 N ZNF618 n/a
18 TRCN0000137631 GAGCTATGTGCTTGGTGTGAA pLKO.1 1551 CDS 100% 4.950 2.970 N ZNF618 n/a
19 TRCN0000138879 GCCAAGAAGATGAACCTCATC pLKO.1 1972 CDS 100% 4.050 2.430 N ZNF618 n/a
20 TRCN0000166364 CACACACACACACACACACAA pLKO.1 2961 3UTR 100% 4.950 2.475 Y KAAG1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_133374.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14357 pDONR223 100% 63.1% 62.9% None 1_951del;1705A>G;2374G>A n/a
2 ccsbBroad304_14357 pLX_304 0% 63.1% 62.9% V5 1_951del;1705A>G;2374G>A n/a
3 TRCN0000477235 GTACTAGGTTAATTAATCAAATCG pLX_317 9.1% 63.1% 62.9% V5 1_951del;1705A>G;2374G>A n/a
Download CSV