Construct: shRNA TRCN0000416234

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153578.2-1206s21c1
DNA Barcode:
TTAACCTAGAAGCTTTCATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nipa1 (233280)
Gene Description:
non imprinted in Prader-Willi/Angelman syndrome 1 homolog (human)
Transcript:
RefSeq NM_153578.2 (CURRENT)
Match location:
Position 1206 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416234)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTAACCTAGAAGCTTTCATTC
Hairpin Sequence:
5'-CCGG-TTAACCTAGAAGCTTTCATTC-CTCGAG-GAATGAAAGCTTCTAGGTTAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTAACCTAGAAGCTTTCATTCCTCGAGGAATGAAAGCTTCTAGGTTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTAACCTAGAAGCTTTCATTCCTCGAGGAATGAAAGCTTCTAGGTTAA-3'

Other clones with same target sequence:

(none)