Construct: shRNA TRCN0000416452

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024650.3-615s21c1
DNA Barcode:
TAACACCCTTCCAGATGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C11orf80 (79703)
Gene Description:
chromosome 11 open reading frame 80
Transcript:
RefSeq NM_024650.3 (CURRENT)
Match location:
Position 615 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416452)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAACACCCTTCCAGATGATTT
Hairpin Sequence:
5'-CCGG-TAACACCCTTCCAGATGATTT-CTCGAG-AAATCATCTGGAAGGGTGTTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAACACCCTTCCAGATGATTTCTCGAGAAATCATCTGGAAGGGTGTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAACACCCTTCCAGATGATTTCTCGAGAAATCATCTGGAAGGGTGTTA-3'

Other clones with same target sequence:

(none)