Construct: shRNA TRCN0000416617

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008310.3-1634s21c1
DNA Barcode:
ATGCAGCTCAGCTCGTAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Htr1f (15557)
Gene Description:
5-hydroxytryptamine (serotonin) receptor 1F
Transcript:
RefSeq NM_008310.3 (CURRENT)
Match location:
Position 1634 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416617)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGCAGCTCAGCTCGTAATAT
Hairpin Sequence:
5'-CCGG-ATGCAGCTCAGCTCGTAATAT-CTCGAG-ATATTACGAGCTGAGCTGCAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGCAGCTCAGCTCGTAATATCTCGAGATATTACGAGCTGAGCTGCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGCAGCTCAGCTCGTAATATCTCGAGATATTACGAGCTGAGCTGCAT-3'

Other clones with same target sequence:

(none)