Construct: shRNA TRCN0000417176

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144931.3-610s21c1
DNA Barcode:
ATTGGAGGATCTACGACTAGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nae1 (234664)
Gene Description:
NEDD8 activating enzyme E1 subunit 1
Transcript:
RefSeq NM_144931.3 (CURRENT)
Match location:
Position 610 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417176)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTGGAGGATCTACGACTAGA
Hairpin Sequence:
5'-CCCG-ATTGGAGGATCTACGACTAGA-CTCGAG-TCTAGTCGTAGATCCTCCAAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTGGAGGATCTACGACTAGACTCGAGTCTAGTCGTAGATCCTCCAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTGGAGGATCTACGACTAGACTCGAGTCTAGTCGTAGATCCTCCAAT-3'

Other clones with same target sequence:

(none)