Transcript: Mouse NM_144931.3

Mus musculus NEDD8 activating enzyme E1 subunit 1 (Nae1), mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Nae1 (234664)
Length:
1808
CDS:
74..1678

Additional Resources:

NCBI RefSeq record:
NM_144931.3
NBCI Gene record:
Nae1 (234664)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_144931.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000413443 GTCTTATGACTTGGACCATAT pLKO_005 664 CDS 100% 10.800 15.120 N Nae1 n/a
2 TRCN0000007240 CCAGGAGTATCTAACTATCAA pLKO.1 1400 CDS 100% 5.625 7.875 N NAE1 n/a
3 TRCN0000293293 CCAGGAGTATCTAACTATCAA pLKO_005 1400 CDS 100% 5.625 7.875 N NAE1 n/a
4 TRCN0000417176 ATTGGAGGATCTACGACTAGA pLKO_005 610 CDS 100% 4.950 6.930 N Nae1 n/a
5 TRCN0000112476 CCATACTCCATGGATTGTGAT pLKO.1 703 CDS 100% 4.950 6.930 N Nae1 n/a
6 TRCN0000112478 GCTTAATAAATGCAACGGCTA pLKO.1 174 CDS 100% 2.160 3.024 N Nae1 n/a
7 TRCN0000112475 GCAGAATGTTTACCGAGAGAA pLKO.1 1099 CDS 100% 4.950 3.960 N Nae1 n/a
8 TRCN0000412912 ATAGAATCTCATCCAGATAAT pLKO_005 587 CDS 100% 13.200 9.240 N Nae1 n/a
9 TRCN0000007238 CCAAGCAGTATTGAAGATATA pLKO.1 917 CDS 100% 13.200 9.240 N NAE1 n/a
10 TRCN0000293362 CCAAGCAGTATTGAAGATATA pLKO_005 917 CDS 100% 13.200 9.240 N NAE1 n/a
11 TRCN0000112479 CCTGATATGATTGCAGATTCA pLKO.1 1061 CDS 100% 4.950 3.465 N Nae1 n/a
12 TRCN0000432960 GAACACCCAGTAATAGAATCT pLKO_005 575 CDS 100% 4.950 3.465 N Nae1 n/a
13 TRCN0000112477 CTAGACAATGATCCTTCATTT pLKO.1 410 CDS 100% 1.320 0.924 N Nae1 n/a
14 TRCN0000007241 GCCATGGAATTCTTACAAGAA pLKO.1 338 CDS 100% 0.000 0.000 N NAE1 n/a
15 TRCN0000293363 GCCATGGAATTCTTACAAGAA pLKO_005 338 CDS 100% 0.000 0.000 N NAE1 n/a
16 TRCN0000436192 GAGAGAACATCTTCAGTCTTA pLKO_005 649 CDS 100% 4.950 2.970 N Nae1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_144931.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02040 pDONR223 100% 92.5% 97% None (many diffs) n/a
2 ccsbBroad304_02040 pLX_304 0% 92.5% 97% V5 (many diffs) n/a
3 TRCN0000470601 GAATTGAGCCACAAATCTGGGACA pLX_317 18.6% 92.5% 97% V5 (many diffs) n/a
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