Construct: shRNA TRCN0000417396

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011139.2-645s21c1
DNA Barcode:
TTCAAGCAGAGACGCATTAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pou2f3 (18988)
Gene Description:
POU domain, class 2, transcription factor 3
Transcript:
RefSeq NM_011139.2 (CURRENT)
Match location:
Position 645 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417396)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCAAGCAGAGACGCATTAAG
Hairpin Sequence:
5'-CCGG-TTCAAGCAGAGACGCATTAAG-CTCGAG-CTTAATGCGTCTCTGCTTGAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCAAGCAGAGACGCATTAAGCTCGAGCTTAATGCGTCTCTGCTTGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCAAGCAGAGACGCATTAAGCTCGAGCTTAATGCGTCTCTGCTTGAA-3'

Other clones with same target sequence:

(none)