Construct: shRNA TRCN0000417478

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172663.4-1700s21c1
DNA Barcode:
GGTCATAATGGACCGAATATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Epc2 (227867)
Gene Description:
enhancer of polycomb homolog 2 (Drosophila)
Transcript:
RefSeq NM_172663.4 (CURRENT)
Match location:
Position 1700 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417478)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTCATAATGGACCGAATATC
Hairpin Sequence:
5'-CCGG-GGTCATAATGGACCGAATATC-CTCGAG-GATATTCGGTCCATTATGACC-TTTTTTTGAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTCATAATGGACCGAATATCCTCGAGGATATTCGGTCCATTATGACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTCATAATGGACCGAATATCCTCGAGGATATTCGGTCCATTATGACC-3'

Other clones with same target sequence:

  1. TRCN0000359790