Construct: shRNA TRCN0000417852

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005418.3-3691s21c1
DNA Barcode:
AGCGAGTGGAGCAGTACTTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DENND2B (6764)
Gene Description:
DENN domain containing 2B
Transcript:
RefSeq NM_005418.3 (CURRENT)
Match location:
Position 3691 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417852)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCGAGTGGAGCAGTACTTAG
Hairpin Sequence:
5'-CCGG-AGCGAGTGGAGCAGTACTTAG-CTCGAG-CTAAGTACTGCTCCACTCGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCGAGTGGAGCAGTACTTAGCTCGAGCTAAGTACTGCTCCACTCGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCGAGTGGAGCAGTACTTAGCTCGAGCTAAGTACTGCTCCACTCGCT-3'

Other clones with same target sequence:

(none)