Construct: shRNA TRCN0000418487

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146257.2-267s21c1
DNA Barcode:
TGGTGACAAGCTTTAGCTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc29a4 (243328)
Gene Description:
solute carrier family 29 (nucleoside transporters), member 4
Transcript:
RefSeq NM_146257.2 (CURRENT)
Match location:
Position 267 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418487)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTGACAAGCTTTAGCTTTG
Hairpin Sequence:
5'-CCGG-TGGTGACAAGCTTTAGCTTTG-CTCGAG-CAAAGCTAAAGCTTGTCACCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTGACAAGCTTTAGCTTTGCTCGAGCAAAGCTAAAGCTTGTCACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTGACAAGCTTTAGCTTTGCTCGAGCAAAGCTAAAGCTTGTCACCA-3'

Other clones with same target sequence:

(none)