Construct: shRNA TRCN0000418521

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021047.2-1672s21c1
DNA Barcode:
GAGTTTATATGGAACACAAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF253 (56242)
Gene Description:
zinc finger protein 253
Transcript:
RefSeq NM_021047.2 (NON-CURRENT)
Match location:
Position 1672 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418521)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTTTATATGGAACACAAAC
Hairpin Sequence:
5'-CCGG-GAGTTTATATGGAACACAAAC-CTCGAG-GTTTGTGTTCCATATAAACTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTTTATATGGAACACAAACCTCGAGGTTTGTGTTCCATATAAACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTTTATATGGAACACAAACCTCGAGGTTTGTGTTCCATATAAACTC-3'

Other clones with same target sequence:

(none)