Construct: shRNA TRCN0000418755

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153108.4-105s21c1
DNA Barcode:
ACATTCGAAACGGAGGCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Defb8 (244334)
Gene Description:
defensin beta 8
Transcript:
RefSeq NM_153108.4 (CURRENT)
Match location:
Position 105 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418755)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACATTCGAAACGGAGGCATAT
Hairpin Sequence:
5'-CCGG-ACATTCGAAACGGAGGCATAT-CTCGAG-ATATGCCTCCGTTTCGAATGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACATTCGAAACGGAGGCATATCTCGAGATATGCCTCCGTTTCGAATGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACATTCGAAACGGAGGCATATCTCGAGATATGCCTCCGTTTCGAATGT-3'

Other clones with same target sequence:

(none)