Construct: shRNA TRCN0000419006

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006261.4-1095s21c1
DNA Barcode:
TGAGGGTCAGCTCAGCGATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PROP1 (5626)
Gene Description:
PROP paired-like homeobox 1
Transcript:
RefSeq NM_006261.4 (CURRENT)
Match location:
Position 1095 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419006)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAGGGTCAGCTCAGCGATTA
Hairpin Sequence:
5'-CCGG-TGAGGGTCAGCTCAGCGATTA-CTCGAG-TAATCGCTGAGCTGACCCTCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAGGGTCAGCTCAGCGATTACTCGAGTAATCGCTGAGCTGACCCTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAGGGTCAGCTCAGCGATTACTCGAGTAATCGCTGAGCTGACCCTCA-3'

Other clones with same target sequence:

(none)