Construct: shRNA TRCN0000419222

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008665.4-1219s21c1
DNA Barcode:
GAAGAAGAAGAGGATGAGGAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Myt1 (17932)
Gene Description:
myelin transcription factor 1
Transcript:
RefSeq NM_008665.4 (CURRENT)
Match location:
Position 1219 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419222)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAAGAAGAGGATGAGGAG
Hairpin Sequence:
5'-CCGG-GAAGAAGAAGAGGATGAGGAG-CTCGAG-CTCCTCATCCTCTTCTTCTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAAGAAGAGGATGAGGAGCTCGAGCTCCTCATCCTCTTCTTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAAGAAGAGGATGAGGAGCTCGAGCTCCTCATCCTCTTCTTCTTC-3'

Other clones with same target sequence:

(none)