Construct: shRNA TRCN0000419414

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172863.4-241s21c1
DNA Barcode:
GAAAGAGGAATGGGCTCTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp697 (242109)
Gene Description:
zinc finger protein 697
Transcript:
RefSeq NM_172863.4 (CURRENT)
Match location:
Position 241 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419414)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGAGGAATGGGCTCTAAT
Hairpin Sequence:
5'-CCGG-GAAAGAGGAATGGGCTCTAAT-CTCGAG-ATTAGAGCCCATTCCTCTTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGAGGAATGGGCTCTAATCTCGAGATTAGAGCCCATTCCTCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGAGGAATGGGCTCTAATCTCGAGATTAGAGCCCATTCCTCTTTC-3'

Other clones with same target sequence:

(none)