Construct: shRNA TRCN0000419505

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002221.3-2952s21c1
DNA Barcode:
TCAAGTGCCACGAGGTCATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ITPKB (3707)
Gene Description:
inositol-trisphosphate 3-kinase B
Transcript:
RefSeq NM_002221.3 (CURRENT)
Match location:
Position 2952 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419505)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAAGTGCCACGAGGTCATTG
Hairpin Sequence:
5'-CCGG-TCAAGTGCCACGAGGTCATTG-CTCGAG-CAATGACCTCGTGGCACTTGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAAGTGCCACGAGGTCATTGCTCGAGCAATGACCTCGTGGCACTTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAAGTGCCACGAGGTCATTGCTCGAGCAATGACCTCGTGGCACTTGA-3'

Other clones with same target sequence:

(none)