Construct: shRNA TRCN0000420517

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177588.2-2837s21c1
DNA Barcode:
GTGCTGCATATCTAGTCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Thnsl1 (208967)
Gene Description:
threonine synthase-like 1 (bacterial)
Transcript:
RefSeq NM_177588.2 (CURRENT)
Match location:
Position 2837 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420517)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCTGCATATCTAGTCATTT
Hairpin Sequence:
5'-CCGG-GTGCTGCATATCTAGTCATTT-CTCGAG-AAATGACTAGATATGCAGCAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCTGCATATCTAGTCATTTCTCGAGAAATGACTAGATATGCAGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCTGCATATCTAGTCATTTCTCGAGAAATGACTAGATATGCAGCAC-3'

Other clones with same target sequence:

(none)