Construct: shRNA TRCN0000421287

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172086.2-173s21c1
DNA Barcode:
TTGACAACAGGGTGCGGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rpl32 (19951)
Gene Description:
ribosomal protein L32
Transcript:
RefSeq NM_172086.2 (CURRENT)
Match location:
Position 173 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421287)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGACAACAGGGTGCGGAGAA
Hairpin Sequence:
5'-CCGG-TTGACAACAGGGTGCGGAGAA-CTCGAG-TTCTCCGCACCCTGTTGTCAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGACAACAGGGTGCGGAGAACTCGAGTTCTCCGCACCCTGTTGTCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGACAACAGGGTGCGGAGAACTCGAGTTCTCCGCACCCTGTTGTCAA-3'

Other clones with same target sequence:

(none)