Transcript: Mouse NM_172086.2

Mus musculus ribosomal protein L32 (Rpl32), mRNA.

Source:
NCBI, updated 2017-05-13
Taxon:
Mus musculus (mouse)
Gene:
Rpl32 (19951)
Length:
504
CDS:
52..459

Additional Resources:

NCBI RefSeq record:
NM_172086.2
NBCI Gene record:
Rpl32 (19951)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_172086.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000421287 TTGACAACAGGGTGCGGAGAA pLKO_005 173 CDS 100% 4.050 2.835 N Rpl32 n/a
2 TRCN0000272382 TGCTGCTGATGTGCAACAAAT pLKO_005 311 CDS 100% 13.200 7.920 N RPL32P18 n/a
3 TRCN0000418166 TGCTGCTGATGTGCAACAAAT pLKO_005 311 CDS 100% 13.200 7.920 N Rpl32 n/a
4 TRCN0000419089 AGGCACCAGTCAGACCGATAT pLKO_005 115 CDS 100% 10.800 6.480 N Rpl32 n/a
5 TRCN0000007955 GCTGATGTGCAACAAATCTTA pLKO.1 315 CDS 100% 5.625 3.375 N RPL32 n/a
6 TRCN0000011879 CAAATCTTACTGTGCTGAGAT pLKO.1 327 CDS 100% 4.950 2.970 N Rpl32 n/a
7 TRCN0000011878 CATCGGTTATGGGAGCAACAA pLKO.1 222 CDS 100% 4.950 2.970 N Rpl32 n/a
8 TRCN0000011881 CTGATGCCCAACATCGGTTAT pLKO.1 211 CDS 100% 10.800 5.400 Y Rpl32 n/a
9 TRCN0000284291 GCAAGTTCCTGGTCCACAATG pLKO_005 275 CDS 100% 10.800 5.400 Y Rpl32l n/a
10 TRCN0000011880 CGCAAGTTCCTGGTCCACAAT pLKO.1 274 CDS 100% 4.950 2.475 Y Rpl32 n/a
11 TRCN0000011882 CTGTGCTGAGATTGCTCACAA pLKO.1 336 CDS 100% 4.950 2.475 Y Rpl32 n/a
12 TRCN0000271055 ATTAAGCGAAACTGGCGGAAA pLKO_005 142 CDS 100% 4.050 2.025 Y Rpl32l n/a
13 TRCN0000117519 CACAATGTCAAGGAGCTGGAA pLKO.1 289 CDS 100% 2.640 1.584 N RPL32P17 n/a
14 TRCN0000271053 CTGATGCCCAACATCGGTTAC pLKO_005 211 CDS 100% 6.000 3.000 Y Rpl32l n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_172086.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01434 pDONR223 100% 91.3% 100% None (many diffs) n/a
2 ccsbBroad304_01434 pLX_304 0% 91.3% 100% V5 (many diffs) n/a
3 TRCN0000465721 TGTTGTTCGTTATACTAAATGTCT pLX_317 95.5% 91.3% 100% V5 (many diffs) n/a
4 ccsbBroadEn_13168 pDONR223 100% 21.7% 23.7% None (many diffs) n/a
5 ccsbBroad304_13168 pLX_304 0% 21.7% 23.7% V5 (many diffs) n/a
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