Construct: shRNA TRCN0000421356
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_016954.2-748s21c1
- DNA Barcode:
- TTGCCCACTGAAGGTGTTAAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TBX22 (50945)
- Gene Description:
- T-box transcription factor 22
- Transcript:
- RefSeq NM_016954.2 (CURRENT)
- Match location:
- Position 748 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000421356)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TTGCCCACTGAAGGTGTTAAA
- Hairpin Sequence:
-
5'-CCGG-TTGCCCACTGAAGGTGTTAAA-CTCGAG-TTTAACACCTTCAGTGGGCAA-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTTGCCCACTGAAGGTGTTAAACTCGAGTTTAACACCTTCAGTGGGCAATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATTGCCCACTGAAGGTGTTAAACTCGAGTTTAACACCTTCAGTGGGCAA-3'
Other clones with same target sequence:
(none)
