Construct: shRNA TRCN0000421584

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009880.3-1133s21c1
DNA Barcode:
GCCCAATGAGAAGTCTCAATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdx1 (12590)
Gene Description:
caudal type homeobox 1
Transcript:
RefSeq NM_009880.3 (CURRENT)
Match location:
Position 1133 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421584)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAATGAGAAGTCTCAATC
Hairpin Sequence:
5'-CCGG-GCCCAATGAGAAGTCTCAATC-CTCGAG-GATTGAGACTTCTCATTGGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAATGAGAAGTCTCAATCCTCGAGGATTGAGACTTCTCATTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAATGAGAAGTCTCAATCCTCGAGGATTGAGACTTCTCATTGGGC-3'

Other clones with same target sequence:

(none)