Construct: shRNA TRCN0000421622

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133256.2-1482s21c1
DNA Barcode:
ATCATTGAAGTTGCCATTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gsx2 (14843)
Gene Description:
GS homeobox 2
Transcript:
RefSeq NM_133256.2 (CURRENT)
Match location:
Position 1482 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421622)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCATTGAAGTTGCCATTAAT
Hairpin Sequence:
5'-CCGG-ATCATTGAAGTTGCCATTAAT-CTCGAG-ATTAATGGCAACTTCAATGAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCATTGAAGTTGCCATTAATCTCGAGATTAATGGCAACTTCAATGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCATTGAAGTTGCCATTAATCTCGAGATTAATGGCAACTTCAATGAT-3'

Other clones with same target sequence:

(none)