Construct: shRNA TRCN0000421650

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026248.3-939s21c1
DNA Barcode:
GCTATTGAAGGCACAAGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4930430A15Rik (67575)
Gene Description:
RIKEN cDNA 4930430A15 gene
Transcript:
RefSeq NM_026248.3 (CURRENT)
Match location:
Position 939 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421650)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATTGAAGGCACAAGTAAA
Hairpin Sequence:
5'-CCGG-GCTATTGAAGGCACAAGTAAA-CTCGAG-TTTACTTGTGCCTTCAATAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATTGAAGGCACAAGTAAACTCGAGTTTACTTGTGCCTTCAATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATTGAAGGCACAAGTAAACTCGAGTTTACTTGTGCCTTCAATAGC-3'

Other clones with same target sequence:

(none)