Construct: shRNA TRCN0000421709

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024650.3-1157s21c1
DNA Barcode:
TTGTGTATGATACCCAATTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C11orf80 (79703)
Gene Description:
chromosome 11 open reading frame 80
Transcript:
RefSeq NM_024650.3 (CURRENT)
Match location:
Position 1157 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421709)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGTGTATGATACCCAATTTG
Hairpin Sequence:
5'-CCGG-TTGTGTATGATACCCAATTTG-CTCGAG-CAAATTGGGTATCATACACAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGTGTATGATACCCAATTTGCTCGAGCAAATTGGGTATCATACACAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGTGTATGATACCCAATTTGCTCGAGCAAATTGGGTATCATACACAA-3'

Other clones with same target sequence:

(none)