Construct: shRNA TRCN0000421713

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018713.2-1678s21c1
DNA Barcode:
TCACATAATCAGACCATATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC30A10 (55532)
Gene Description:
solute carrier family 30 member 10
Transcript:
RefSeq NM_018713.2 (CURRENT)
Match location:
Position 1678 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421713)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCACATAATCAGACCATATAG
Hairpin Sequence:
5'-CCGG-TCACATAATCAGACCATATAG-CTCGAG-CTATATGGTCTGATTATGTGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCACATAATCAGACCATATAGCTCGAGCTATATGGTCTGATTATGTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCACATAATCAGACCATATAGCTCGAGCTATATGGTCTGATTATGTGA-3'

Other clones with same target sequence:

(none)