Construct: shRNA TRCN0000421923
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_153140.2-1867s21c1
- DNA Barcode:
- TTTCTGCTATGCGCCCTATTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Rab11fip3 (215445)
- Gene Description:
- RAB11 family interacting protein 3 (class II)
- Transcript:
- RefSeq NM_153140.2 (CURRENT)
- Match location:
- Position 1867 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000421923)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TTTCTGCTATGCGCCCTATTA
- Hairpin Sequence:
-
5'-CCGG-TTTCTGCTATGCGCCCTATTA-CTCGAG-TAATAGGGCGCATAGCAGAAA-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTTTCTGCTATGCGCCCTATTACTCGAGTAATAGGGCGCATAGCAGAAATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATTTCTGCTATGCGCCCTATTACTCGAGTAATAGGGCGCATAGCAGAAA-3'
Other clones with same target sequence:
(none)
