Construct: shRNA TRCN0000422183

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010445.2-629s21c1
DNA Barcode:
TCGATCTGAAGCGCTACCTGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hmx1 (15371)
Gene Description:
H6 homeobox 1
Transcript:
RefSeq NM_010445.2 (CURRENT)
Match location:
Position 629 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422183)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGATCTGAAGCGCTACCTGA
Hairpin Sequence:
5'-CCGG-TCGATCTGAAGCGCTACCTGA-CTCGAG-TCAGGTAGCGCTTCAGATCGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGATCTGAAGCGCTACCTGACTCGAGTCAGGTAGCGCTTCAGATCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGATCTGAAGCGCTACCTGACTCGAGTCAGGTAGCGCTTCAGATCGA-3'

Other clones with same target sequence:

(none)