Construct: shRNA TRCN0000422389

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173774.3-1717s21c1
DNA Barcode:
CTGTTCCACCATCTACAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc45a1 (242773)
Gene Description:
solute carrier family 45, member 1
Transcript:
RefSeq NM_173774.3 (CURRENT)
Match location:
Position 1717 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422389)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTTCCACCATCTACAATAT
Hairpin Sequence:
5'-CCGG-CTGTTCCACCATCTACAATAT-CTCGAG-ATATTGTAGATGGTGGAACAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTTCCACCATCTACAATATCTCGAGATATTGTAGATGGTGGAACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTTCCACCATCTACAATATCTCGAGATATTGTAGATGGTGGAACAG-3'

Other clones with same target sequence:

(none)