Construct: shRNA TRCN0000422808

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080788.3-4284s21c1
DNA Barcode:
GATAGGAAATAGAATGGGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ttbk2 (140810)
Gene Description:
tau tubulin kinase 2
Transcript:
RefSeq NM_080788.3 (CURRENT)
Match location:
Position 4284 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422808)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATAGGAAATAGAATGGGATT
Hairpin Sequence:
5'-CCGG-GATAGGAAATAGAATGGGATT-CTCGAG-AATCCCATTCTATTTCCTATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATAGGAAATAGAATGGGATTCTCGAGAATCCCATTCTATTTCCTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATAGGAAATAGAATGGGATTCTCGAGAATCCCATTCTATTTCCTATC-3'

Other clones with same target sequence:

(none)