Transcript: Mouse NM_080788.3

Mus musculus tau tubulin kinase 2 (Ttbk2), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Ttbk2 (140810)
Length:
10906
CDS:
52..3990

Additional Resources:

NCBI RefSeq record:
NM_080788.3
NBCI Gene record:
Ttbk2 (140810)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_080788.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000417231 GTAAGGAAGCTACGTTCTATT pLKO_005 1576 CDS 100% 13.200 18.480 N Ttbk2 n/a
2 TRCN0000431403 TGGCACTATGATGACGAATAT pLKO_005 1771 CDS 100% 13.200 18.480 N Ttbk2 n/a
3 TRCN0000230525 TTCGAGGGACAGTTCGTTATG pLKO_005 815 CDS 100% 10.800 15.120 N TTBK2 n/a
4 TRCN0000429973 AGCCAGGCTACGCAGATATAA pLKO_005 3522 CDS 100% 15.000 10.500 N Ttbk2 n/a
5 TRCN0000023789 GCACCTTTCTTGACCATATTT pLKO.1 1025 CDS 100% 15.000 10.500 N Ttbk2 n/a
6 TRCN0000023791 CCAGCTTCTAACATCCGTGTT pLKO.1 1077 CDS 100% 4.050 2.835 N Ttbk2 n/a
7 TRCN0000422808 GATAGGAAATAGAATGGGATT pLKO_005 4284 3UTR 100% 4.050 2.835 N Ttbk2 n/a
8 TRCN0000023792 CTGGTCAAAGAAAGATGGAAA pLKO.1 304 CDS 100% 4.950 2.970 N Ttbk2 n/a
9 TRCN0000023790 GCTGTGTTGAAGAAACTGCAA pLKO.1 454 CDS 100% 2.640 1.584 N Ttbk2 n/a
10 TRCN0000106535 GCCTGGTCTACAAAGTGAGTT pLKO.1 7465 3UTR 100% 4.950 2.475 Y Gad2 n/a
11 TRCN0000003232 TGCATGTGTGTCCCTGTACTT pLKO.1 4049 3UTR 100% 4.950 2.970 N TTBK2 n/a
12 TRCN0000054408 ACGCCTTTAATCCCAGCACTT pLKO.1 7406 3UTR 100% 4.050 2.025 Y Mtif2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_080788.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488941 CCCTATTACCTGCGAATGTTGACG pLX_317 10.2% 84.2% 85% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000489416 TCTGCCCAAAACTGACGATTGCGA pLX_317 10% 84.2% 85% V5 (many diffs) n/a
3 TRCN0000489781 CGGGCGTAAACGGCTAGCTTTTAC pLX_317 10.9% 81.7% 82.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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