Construct: shRNA TRCN0000422907

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008713.4-3893s21c1
DNA Barcode:
CTGGAGTATCTTACGTTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nos3 (18127)
Gene Description:
nitric oxide synthase 3, endothelial cell
Transcript:
RefSeq NM_008713.4 (CURRENT)
Match location:
Position 3893 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422907)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGAGTATCTTACGTTTAAA
Hairpin Sequence:
5'-CCGG-CTGGAGTATCTTACGTTTAAA-CTCGAG-TTTAAACGTAAGATACTCCAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGAGTATCTTACGTTTAAACTCGAGTTTAAACGTAAGATACTCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGAGTATCTTACGTTTAAACTCGAGTTTAAACGTAAGATACTCCAG-3'

Other clones with same target sequence:

(none)