Construct: shRNA TRCN0000423542

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172403.2-2041s21c1
DNA Barcode:
ACAGAGACTCTGAGTCTAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2810021J22Rik (69944)
Gene Description:
RIKEN cDNA 2810021J22 gene
Transcript:
RefSeq NM_172403.2 (CURRENT)
Match location:
Position 2041 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423542)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGAGACTCTGAGTCTAAAG
Hairpin Sequence:
5'-CCGG-ACAGAGACTCTGAGTCTAAAG-CTCGAG-CTTTAGACTCAGAGTCTCTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGAGACTCTGAGTCTAAAGCTCGAGCTTTAGACTCAGAGTCTCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGAGACTCTGAGTCTAAAGCTCGAGCTTTAGACTCAGAGTCTCTGT-3'

Other clones with same target sequence:

(none)