Construct: shRNA TRCN0000423951

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007421.2-1174s21c1
DNA Barcode:
GATGTCCTGAGCGAGATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adssl1 (11565)
Gene Description:
adenylosuccinate synthetase like 1
Transcript:
RefSeq NM_007421.2 (CURRENT)
Match location:
Position 1174 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423951)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGTCCTGAGCGAGATTAAA
Hairpin Sequence:
5'-CCGG-GATGTCCTGAGCGAGATTAAA-CTCGAG-TTTAATCTCGCTCAGGACATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGTCCTGAGCGAGATTAAACTCGAGTTTAATCTCGCTCAGGACATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGTCCTGAGCGAGATTAAACTCGAGTTTAATCTCGCTCAGGACATC-3'

Other clones with same target sequence:

(none)