Construct: shRNA TRCN0000424049

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172540.2-1839s21c1
DNA Barcode:
TAACTCCTTGAACCTGAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trmt2b (215201)
Gene Description:
TRM2 tRNA methyltransferase 2B
Transcript:
RefSeq NM_172540.2 (CURRENT)
Match location:
Position 1839 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424049)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAACTCCTTGAACCTGAATTT
Hairpin Sequence:
5'-CCGG-TAACTCCTTGAACCTGAATTT-CTCGAG-AAATTCAGGTTCAAGGAGTTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAACTCCTTGAACCTGAATTTCTCGAGAAATTCAGGTTCAAGGAGTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAACTCCTTGAACCTGAATTTCTCGAGAAATTCAGGTTCAAGGAGTTA-3'

Other clones with same target sequence:

(none)