Construct: shRNA TRCN0000424052

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002260.3-703s21c1
DNA Barcode:
GAAGTAAAGCATTTGCGTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KLRC2 (3822)
Gene Description:
killer cell lectin like receptor C2
Transcript:
RefSeq NM_002260.3 (CURRENT)
Match location:
Position 703 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424052)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTAAAGCATTTGCGTTTG
Hairpin Sequence:
5'-CCGG-GAAGTAAAGCATTTGCGTTTG-CTCGAG-CAAACGCAAATGCTTTACTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTAAAGCATTTGCGTTTGCTCGAGCAAACGCAAATGCTTTACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTAAAGCATTTGCGTTTGCTCGAGCAAACGCAAATGCTTTACTTC-3'

Other clones with same target sequence:

(none)