Construct: shRNA TRCN0000424246

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017461.2-1029s21c1
DNA Barcode:
GATTCGCGAGAAGGACGAAGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sept1 (54204)
Gene Description:
septin 1
Transcript:
RefSeq NM_017461.2 (CURRENT)
Match location:
Position 1029 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424246)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTCGCGAGAAGGACGAAGA
Hairpin Sequence:
5'-CCGG-GATTCGCGAGAAGGACGAAGA-CTCGAG-TCTTCGTCCTTCTCGCGAATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTCGCGAGAAGGACGAAGACTCGAGTCTTCGTCCTTCTCGCGAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTCGCGAGAAGGACGAAGACTCGAGTCTTCGTCCTTCTCGCGAATC-3'

Other clones with same target sequence:

(none)