Construct: shRNA TRCN0000424254

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172438.3-1297s21c1
DNA Barcode:
CAAATCCAGCCAATCAGTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Thoc5 (107829)
Gene Description:
THO complex 5
Transcript:
RefSeq NM_172438.3 (CURRENT)
Match location:
Position 1297 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424254)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAATCCAGCCAATCAGTATC
Hairpin Sequence:
5'-CCGG-CAAATCCAGCCAATCAGTATC-CTCGAG-GATACTGATTGGCTGGATTTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAATCCAGCCAATCAGTATCCTCGAGGATACTGATTGGCTGGATTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAATCCAGCCAATCAGTATCCTCGAGGATACTGATTGGCTGGATTTG-3'

Other clones with same target sequence:

(none)