Construct: shRNA TRCN0000424557

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003535.2-160s21c1
DNA Barcode:
CGTTATCAGAAGTCGACTGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H3C12 (8356)
Gene Description:
H3 clustered histone 12
Transcript:
RefSeq NM_003535.2 (CURRENT)
Match location:
Position 160 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424557)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTATCAGAAGTCGACTGAG
Hairpin Sequence:
5'-CCGG-CGTTATCAGAAGTCGACTGAG-CTCGAG-CTCAGTCGACTTCTGATAACG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTATCAGAAGTCGACTGAGCTCGAGCTCAGTCGACTTCTGATAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTATCAGAAGTCGACTGAGCTCGAGCTCAGTCGACTTCTGATAACG-3'

Other clones with same target sequence:

(none)