Construct: shRNA TRCN0000425090

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028732.4-1141s21c1
DNA Barcode:
GATATGTGCACTTGATCTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vsir (74048)
Gene Description:
V-set immunoregulatory receptor
Transcript:
RefSeq NM_028732.4 (CURRENT)
Match location:
Position 1141 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425090)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATATGTGCACTTGATCTATG
Hairpin Sequence:
5'-CCGG-GATATGTGCACTTGATCTATG-CTCGAG-CATAGATCAAGTGCACATATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATATGTGCACTTGATCTATGCTCGAGCATAGATCAAGTGCACATATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATATGTGCACTTGATCTATGCTCGAGCATAGATCAAGTGCACATATC-3'

Other clones with same target sequence:

(none)