Construct: shRNA TRCN0000425614

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030971.3-675s21c1
DNA Barcode:
GGCCAAGTATGTGTATGACTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SFXN3 (81855)
Gene Description:
sideroflexin 3
Transcript:
RefSeq NM_030971.3 (CURRENT)
Match location:
Position 675 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425614)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCCAAGTATGTGTATGACTC
Hairpin Sequence:
5'-CCGG-GGCCAAGTATGTGTATGACTC-CTCGAG-GAGTCATACACATACTTGGCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCCAAGTATGTGTATGACTCCTCGAGGAGTCATACACATACTTGGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCCAAGTATGTGTATGACTCCTCGAGGAGTCATACACATACTTGGCC-3'

Other clones with same target sequence:

(none)