Construct: shRNA TRCN0000425655

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003548.2-285s21c1
DNA Barcode:
ATGTGGTGTACGCGCTCAAGC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H4C14 (8370)
Gene Description:
H4 clustered histone 14
Transcript:
RefSeq NM_003548.2 (CURRENT)
Match location:
Position 285 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425655)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTGGTGTACGCGCTCAAGC
Hairpin Sequence:
5'-CCGG-ATGTGGTGTACGCGCTCAAGC-CTCGAG-GCTTGAGCGCGTACACCACAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTGGTGTACGCGCTCAAGCCTCGAGGCTTGAGCGCGTACACCACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTGGTGTACGCGCTCAAGCCTCGAGGCTTGAGCGCGTACACCACAT-3'

Other clones with same target sequence:

(none)