Transcript: Human NM_003548.2

Homo sapiens H4 clustered histone 14 (H4C14), mRNA.

Source:
NCBI, updated 2019-07-25
Taxon:
Homo sapiens (human)
Gene:
H4C14 (8370)
Length:
396
CDS:
29..340

Additional Resources:

NCBI RefSeq record:
NM_003548.2
NBCI Gene record:
H4C14 (8370)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_003548.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000437392 AGCGGATCTCTGGCCTCATTT pLKO_005 162 CDS 100% 13.200 6.600 Y H4C14 n/a
2 TRCN0000256282 GCGGATCTCTGGCCTCATTTA pLKO_005 163 CDS 100% 13.200 6.600 Y H4C15 n/a
3 TRCN0000256285 AGGTCTTGAGAGACAACATTC pLKO_005 90 CDS 100% 10.800 5.400 Y H4C15 n/a
4 TRCN0000256283 TGAAGGTGTTCCTGGAGAATG pLKO_005 204 CDS 100% 10.800 5.400 Y H4C15 n/a
5 TRCN0000106780 CAAGGTCTTGAGAGACAACAT pLKO.1 88 CDS 100% 4.950 2.475 Y H4C14 n/a
6 TRCN0000179971 CAAGGTCTTGAGAGACAACAT pLKO.1 88 CDS 100% 4.950 2.475 Y H4C15 n/a
7 TRCN0000181157 CTGAAGGTGTTCCTGGAGAAT pLKO.1 203 CDS 100% 4.950 2.475 Y H4C15 n/a
8 TRCN0000179301 GTGTTCCTGGAGAATGTGATT pLKO.1 209 CDS 100% 4.950 2.475 Y H4C15 n/a
9 TRCN0000092704 GCTGAAGGTGTTCCTGGAGAA pLKO.1 202 CDS 100% 4.050 2.025 Y Hist1h4h n/a
10 TRCN0000423699 ACAACATTCAGGGCATCACCA pLKO_005 102 CDS 100% 2.640 1.320 Y H4C14 n/a
11 TRCN0000256281 ATGTGATTCGGGACGCAGTCA pLKO_005 222 CDS 100% 2.640 1.320 Y H4C15 n/a
12 TRCN0000256284 GACAACATTCAGGGCATCACC pLKO_005 101 CDS 100% 2.640 1.320 Y H4C15 n/a
13 TRCN0000425655 ATGTGGTGTACGCGCTCAAGC pLKO_005 285 CDS 100% 1.350 0.675 Y H4C14 n/a
14 TRCN0000434434 GACCGTCACAGCCATGGATGT pLKO_005 268 CDS 100% 1.350 0.675 Y H4C14 n/a
15 TRCN0000092706 CGGTGTGCTGAAGGTGTTCCT pLKO.1 196 CDS 100% 0.880 0.440 Y Hist1h4h n/a
16 TRCN0000106784 GATTCGGGACGCAGTCACCTA pLKO.1 226 CDS 100% 0.880 0.440 Y H4C14 n/a
17 TRCN0000106782 CCTGCCATTCGGCGTCTAGCT pLKO.1 125 CDS 100% 0.000 0.000 Y H4C14 n/a
18 TRCN0000106781 CGCAGTCACCTACACCGAGCA pLKO.1 235 CDS 100% 0.000 0.000 Y H4C14 n/a
19 TRCN0000106783 CGCCACCGCAAGGTCTTGAGA pLKO.1 80 CDS 100% 0.000 0.000 Y H4C14 n/a
20 TRCN0000106728 GTGTTCCTGGAGAATGTGATA pLKO.1 209 CDS 100% 4.950 2.475 Y H4C6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_003548.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05703 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_05703 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000478553 TTTATTGTGCGATTTATCCGATTA pLX_317 82.9% 100% 100% V5 n/a
4 ccsbBroadEn_01912 pDONR223 100% 85.8% 100% None (many diffs) n/a
5 ccsbBroad304_01912 pLX_304 0% 85.8% 100% V5 (many diffs) n/a
6 TRCN0000472437 ACTCCACAACGATCTTTCCTTCAT pLX_317 100% 85.8% 100% V5 (many diffs) n/a
7 ccsbBroadEn_07216 pDONR223 100% 85.7% 100% None (many diffs) n/a
8 ccsbBroad304_07216 pLX_304 0% 85.7% 100% V5 (many diffs) n/a
9 TRCN0000468808 CGAAACCTGTGAAAGAAATAGCCA pLX_317 100% 85.7% 100% V5 (many diffs) n/a
10 ccsbBroadEn_01883 pDONR223 100% 85.4% 100% None (many diffs) n/a
11 ccsbBroad304_01883 pLX_304 0% 85.4% 100% V5 (many diffs) n/a
12 TRCN0000470786 AAACACGGTAGAGCCAAGTCGACG pLX_317 100% 85.4% 100% V5 (many diffs) n/a
13 ccsbBroadEn_01910 pDONR223 100% 84.4% 100% None (many diffs) n/a
14 ccsbBroad304_01910 pLX_304 0% 84.4% 100% V5 (many diffs) n/a
15 TRCN0000470331 GGAGTACAACATTTCGGGTCCGAA pLX_317 100% 84.4% 100% V5 (many diffs) n/a
16 ccsbBroadEn_01911 pDONR223 100% 84.1% 100% None (many diffs) n/a
17 ccsbBroad304_01911 pLX_304 0% 84.1% 100% V5 (many diffs) n/a
18 TRCN0000472587 AGGCTCCAGTCGACTTCTCCCACC pLX_317 100% 84.1% 100% V5 (many diffs) n/a
19 ccsbBroadEn_01916 pDONR223 100% 84.1% 100% None (many diffs) n/a
20 ccsbBroad304_01916 pLX_304 0% 84.1% 100% V5 (many diffs) n/a
21 TRCN0000468855 AAAAGGGCACCCCGTTGTGATCCC pLX_317 100% 84.1% 100% V5 (many diffs) n/a
22 ccsbBroadEn_11268 pDONR223 100% 83.1% 100% None (many diffs) n/a
23 ccsbBroad304_11268 pLX_304 0% 83.1% 100% V5 (many diffs) n/a
24 TRCN0000473699 AAATTGTTGTTGTCACATGCCGTA pLX_317 100% 83.1% 100% V5 (many diffs) n/a
25 ccsbBroadEn_07227 pDONR223 100% 82.8% 99% None (many diffs) n/a
26 ccsbBroad304_07227 pLX_304 0% 82.8% 99% V5 (many diffs) n/a
27 TRCN0000466667 ACAGCTATGCTGGGTCCGATAGTT pLX_317 69.3% 82.8% 99% V5 (many diffs) n/a
28 ccsbBroadEn_01915 pDONR223 100% 82.2% 100% None (many diffs) n/a
29 ccsbBroad304_01915 pLX_304 0% 82.2% 100% V5 (many diffs) n/a
30 TRCN0000475279 TATAGCGGGACGTGTAGAGTAGCT pLX_317 27.6% 82.2% 100% V5 (many diffs) n/a
31 ccsbBroadEn_01914 pDONR223 100% 81.2% 100% None (many diffs) n/a
32 ccsbBroad304_01914 pLX_304 0% 81.2% 100% V5 (many diffs) n/a
33 TRCN0000470478 ACCAGTGCAGTTCTATCGAGTATT pLX_317 100% 81.2% 100% V5 (many diffs) n/a
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