Construct: shRNA TRCN0000425766

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019098.4-2874s21c1
DNA Barcode:
GGCTAATTGTTCTAGGTTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CNGB3 (54714)
Gene Description:
cyclic nucleotide gated channel subunit beta 3
Transcript:
RefSeq NM_019098.4 (CURRENT)
Match location:
Position 2874 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000425766)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCTAATTGTTCTAGGTTTAT
Hairpin Sequence:
5'-CCGG-GGCTAATTGTTCTAGGTTTAT-CTCGAG-ATAAACCTAGAACAATTAGCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCTAATTGTTCTAGGTTTATCTCGAGATAAACCTAGAACAATTAGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCTAATTGTTCTAGGTTTATCTCGAGATAAACCTAGAACAATTAGCC-3'

Other clones with same target sequence:

(none)