Construct: shRNA TRCN0000426093

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008314.2-1609s21c1
DNA Barcode:
TTGTAGCTCAGTGGGTTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Htr5a (15563)
Gene Description:
5-hydroxytryptamine (serotonin) receptor 5A
Transcript:
RefSeq NM_008314.2 (CURRENT)
Match location:
Position 1609 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426093)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGTAGCTCAGTGGGTTATAT
Hairpin Sequence:
5'-CCGG-TTGTAGCTCAGTGGGTTATAT-CTCGAG-ATATAACCCACTGAGCTACAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGTAGCTCAGTGGGTTATATCTCGAGATATAACCCACTGAGCTACAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGTAGCTCAGTGGGTTATATCTCGAGATATAACCCACTGAGCTACAA-3'

Other clones with same target sequence:

(none)