Construct: shRNA TRCN0000426162

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024703.1-1168s21c1
DNA Barcode:
GTCAAATCGGAAGCGGTTAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mctp2 (244049)
Gene Description:
multiple C2 domains, transmembrane 2
Transcript:
RefSeq NM_001024703.1 (CURRENT)
Match location:
Position 1168 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426162)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAAATCGGAAGCGGTTAAG
Hairpin Sequence:
5'-CCGG-GTCAAATCGGAAGCGGTTAAG-CTCGAG-CTTAACCGCTTCCGATTTGAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAAATCGGAAGCGGTTAAGCTCGAGCTTAACCGCTTCCGATTTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAAATCGGAAGCGGTTAAGCTCGAGCTTAACCGCTTCCGATTTGAC-3'

Other clones with same target sequence:

(none)