Construct: shRNA TRCN0000426343

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175006.2-1138s21c1
DNA Barcode:
ATGCACAAGGACAGATTATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pou6f2 (218030)
Gene Description:
POU domain, class 6, transcription factor 2
Transcript:
RefSeq NM_175006.2 (CURRENT)
Match location:
Position 1138 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426343)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGCACAAGGACAGATTATTG
Hairpin Sequence:
5'-CCGG-ATGCACAAGGACAGATTATTG-CTCGAG-CAATAATCTGTCCTTGTGCAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGCACAAGGACAGATTATTGCTCGAGCAATAATCTGTCCTTGTGCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGCACAAGGACAGATTATTGCTCGAGCAATAATCTGTCCTTGTGCAT-3'

Other clones with same target sequence:

(none)