Construct: shRNA TRCN0000426363

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145537.2-1865s21c1
DNA Barcode:
CCTCATAGCCACTGGATAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Edem2 (108687)
Gene Description:
ER degradation enhancer, mannosidase alpha-like 2
Transcript:
RefSeq NM_145537.2 (CURRENT)
Match location:
Position 1865 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426363)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATAGCCACTGGATAATT
Hairpin Sequence:
5'-CCGG-CCTCATAGCCACTGGATAATT-CTCGAG-AATTATCCAGTGGCTATGAGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATAGCCACTGGATAATTCTCGAGAATTATCCAGTGGCTATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATAGCCACTGGATAATTCTCGAGAATTATCCAGTGGCTATGAGG-3'

Other clones with same target sequence:

(none)