Construct: shRNA TRCN0000426380

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080470.1-1793s21c1
DNA Barcode:
TAAGATGATGATCGATGTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smc1b (140557)
Gene Description:
structural maintenance of chromosomes 1B
Transcript:
RefSeq NM_080470.1 (CURRENT)
Match location:
Position 1793 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426380)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAGATGATGATCGATGTTAT
Hairpin Sequence:
5'-CCGG-TAAGATGATGATCGATGTTAT-CTCGAG-ATAACATCGATCATCATCTTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAGATGATGATCGATGTTATCTCGAGATAACATCGATCATCATCTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAGATGATGATCGATGTTATCTCGAGATAACATCGATCATCATCTTA-3'

Other clones with same target sequence:

(none)