Construct: shRNA TRCN0000426460

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011836.3-1100s21c1
DNA Barcode:
CGTTTGACAGGGAGCTCTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lamc3 (23928)
Gene Description:
laminin gamma 3
Transcript:
RefSeq NM_011836.3 (CURRENT)
Match location:
Position 1100 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426460)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTTGACAGGGAGCTCTATC
Hairpin Sequence:
5'-CCGG-CGTTTGACAGGGAGCTCTATC-CTCGAG-GATAGAGCTCCCTGTCAAACG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTTGACAGGGAGCTCTATCCTCGAGGATAGAGCTCCCTGTCAAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTTGACAGGGAGCTCTATCCTCGAGGATAGAGCTCCCTGTCAAACG-3'

Other clones with same target sequence:

(none)