Construct: shRNA TRCN0000426595

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011784.3-1675s21c1
DNA Barcode:
ATAAATGAGGGAAGGTTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aplnr (23796)
Gene Description:
apelin receptor
Transcript:
RefSeq NM_011784.3 (CURRENT)
Match location:
Position 1675 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426595)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATAAATGAGGGAAGGTTATAT
Hairpin Sequence:
5'-CCGG-ATAAATGAGGGAAGGTTATAT-CTCGAG-ATATAACCTTCCCTCATTTAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATAAATGAGGGAAGGTTATATCTCGAGATATAACCTTCCCTCATTTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATAAATGAGGGAAGGTTATATCTCGAGATATAACCTTCCCTCATTTAT-3'

Other clones with same target sequence:

(none)